Jian-Ping Jin, M.D., Ph.D.
Professor and the William D. Traitel
Endowed Chair of Physiology
Lab: 5344 Scott Hall
Office: 5374 Scott Hall
540 E. Canfield, Detroit, MI 48201
313-577-1520, FAX 313-577-5494
jjin@med.wayne.edu

Editors in Chief, Archives of Biochemistry and Biophysics

Established in 1993, my lab is interested in the regulation and structure-function relationships of contractile and cytoskeleton proteins. Muscle (cardiac, skeletal and smooth) contraction and non-muscle cell motility play vital roles in physiological activities and pathological conditions. Our research is focused on actin thin filament-associated regulatory proteins: troponin and calponin, to study their regulation and structure-function relationships. Molecular biology and genetic approaches are used to investigate protein isoform evolution and expression as well as to provide engineered protein constructs for functional characterization. Biochemical, biophysical and immunochemical methods are used in studying protein structure and function. Cell culture systems and transgenic/gene knock-out/knock-in mouse models are developed for integrative functional characterizations at cellular, organ and whole animal levels.

My lab will have an opening for a new PhD student and possibly an MS student beginning Spring/Summer 2016)

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ONGOING PROJECTS:
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Regulation and function of troponin T isoforms: Biochemical and biophysical studies are performed to investigate the functional significance of various troponin T isoforms in the Ca2+-regulation of cardiac and skeletal muscle contraction and diseases. The studies are focused on the abnormal cardiac troponin T splicing variants found in dilated cardiomyopathy and a slow skeletal muscle troponin T mutation that causes a lethal type of nemaline myopathy. This line of study is currently funded by NIH grants R01HL127691 and R01AR048816.

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Post-translational regulation of troponin during muscle adaptation and diseases: Proteolytic modification of troponin I and troponin T is studied for functional significance and therapeutic values in the Ca2+-regulation of myocardial contraction in adaptation to hemodynamic stresses, myocardial ischemia-reperfusion injury, heart failure, and skeletal muscle fatigue. This line of study is currently funded by NIH grants R01HL98945 and R01HL127691.

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Mechanical tension regulation and function of calponin: To study calponin's function as a troponin analog in smooth muscle and non-muscle cells, we are investigating their role in the fine-tuning of smooth muscle contractility, non-muscle motility, and the function of actin cytoskeleton during development, tissue remodeling and cytokinesis. The study is focused on mechanical tension regulated calponin gene expression and protein degradation in epithelial, endothelial, fibroblast, macrophages and smooth muscle cells.

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Protein engineering and immunological detection of biomarkers: Advanced technologies are used to generate novel tools and develop new diagnostic methods for myocardial and infectious diseases, as well as industrial production of recombinant proteins.  This line of research is currently supported by a collaboration contract and NIH grant R21AI116659.

 

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SELECTED PUBLICATIONS - (Last three years)
A complete list of Dr. Jin's publications can be found at PubMed-Jin

  1. Troponin: Informative Diagnostic Marker. J.-P. Jin Ed., Nova Science Publishers, 2014

  2. Wei H, Jin JP. N-terminal truncations of cardiac troponin I and cardiac troponin T produce distinct effects on contractility and calcium homeostasis in adult cardiomyocytes. Am J Physiol Cell Physiol. 2014 Dec 17. PMID: 25518962

  3. Hines PC, Gao X, White JC, D'Agostino A, Jin JP. A novel role of h2-calponin in regulating whole blood thrombosis and platelet adhesion during physiologic flow. Physiol Rep. 2014 Dec 3;2(12). PMID: 25472609

  4. Feng HZ, Chen G, Nan C, Huang X, Jin JP. Abnormal splicing in the N-terminal variable region of cardiac troponin T impairs systolic function of the heart with preserved Frank-Starling compensation. Physiol Rep. 2014 Sep 4;2(9). pii: e12139. PMID: 25194024

  5. Moazzem Hossain M, Wang X, Bergan RC, Jin JP. Diminished expression of h2-calponin in prostate cancer cells promotes cell proliferation, migration and the dependence of cell adhesion on substrate stiffness. FEBS Open Bio. 2014 Jun 24;4:627-36. PMID: 25161871

  6. Debald M, Jin JP, Linke A, Walgenbach KJ, Rauch P, Zellmer A, Fimmers R, Kuhn W, Hartmann G, Walgenbach-Brünagel G. Calponin-h2: a potential serum marker for the early detection of human breast cancer? Tumour Biol. 2014 Nov;35(11):11121-7. PMID: 25099617

  7. Corpeno R, Dworkin B, Cacciani N, Salah H, Bergman HM, Ravara B, Vitadello M, Gorza L, Gustafson AM, Hedström Y, Petersson J, Feng HZ, Jin JP, Iwamoto H, Yagi N, Artemenko K, Bergquist J, Larsson L. Time course analysis of mechanical ventilation-induced diaphragm contractile muscle dysfunction in the rat. J Physiol. 2014 Sep 1;592(Pt 17):3859-80. PMID: 25015920

  8. Wei H, Jin JP. A dominantly negative mutation in cardiac troponin I at the interface with troponin T causes early remodeling in ventricular cardiomyocytes. Am J Physiol Cell Physiol. 2014 Aug 15;307(4):C338-48. PMID: 24898585

  9. Sheng JJ, Jin JP. Gene regulation, alternative splicing, and posttranslational modification of troponin subunits in cardiac development and adaptation: a focused review. Front Physiol. 2014 Apr 30;5:165. PMID: 24817852

  10. Liu R, Feng HZ, Jin JP. Physiological contractility of cardiomyocytes in the wall of mouse and rat azygos vein.  Am J Physiol Cell Physiol. 2014 Apr 1;306(7):C697-704. PMID: 24477237

  11. Wei B, Lu Y, Jin JP. Deficiency of slow skeletal muscle troponin T causes atrophy of type I slow fibres and decreases tolerance to fatigue. J Physiol. 2014 Mar 15;592(Pt 6):1367-80. PMID: 24445317

  12. Huang QQ, Birkett R, Koessler RE, Cuda CM, Haines GK 3rd, Jin JP, Perlman H, Pope RM.  Fas signaling in macrophages promotes chronicity in K/BxN serum-induced arthritis. Arthritis Rheumatol. 2014 Jan;66(1):68-77. PMID: 24431281

  13. Zhang T, Choi SJ, Wang ZM, Birbrair A, Messi ML, Jin JP, Marsh AP, Nicklas B, Delbono O. Human slow troponin T (TNNT1) pre-mRNA alternative splicing is an indicator of skeletal muscle response to resistance exercise in older adults. J Gerontol A Biol Sci Med Sci. 2014 Dec;69(12):1437-47. PMID: 24368775

  14. Wei H, Jin JP. NH2-terminal truncations of cardiac troponin I and cardiac troponin T produce distinct effects on contractility and calcium homeostasis in adult cardiomyocytes. Am J Physiol Cell Physiol. 2015 Mar 1;308(5):C397-404. PMID: 25518962

  15. Liu R, Jin JP. Calponin: A mechanical tension-modulated regulator of cytoskeleton and cell motility. Current Topics in Biochemical Research, 2015, 16: 1–15.

  16. Akhter S, Jin JP. Distinct conformational and functional effects of two adjacent pathogenic mutations in cardiac troponin I at the interface with troponin T. FEBS Open Bio. 2015 Jan 13;5:64-75. PMID: 25685665

  17. Amarasinghe C, Jin JP. The N-terminal hypervariable region of muscle type isoforms of troponin T differentially modulates the affinity of tropomyosin-binding site 1. Biochemistry. 2015 Jun 10. PMID: 26024675

  18. Martins AS, Parvatiyar MS, Feng HZ, Bos JM, Gonzalez-Martinez D, Vukmirovic M, Turna RS, Sanchez-Gonzalez MA, Badger CD, Zorio DA, Singh RK, Wang Y, Jin JP, Ackerman MJ, Pinto JR. In vivo Analysis of Troponin C Knock-in (A8V) Mice: Evidence that TNNC1 Is a Hypertrophic Cardiomyopathy Susceptibility Gene. Circ Cardiovasc Genet. 2015 Aug 24. PMID: 26304555

  19. Wei B, Wei H, Jin JP. Dysferlin deficiency blunts β-adrenergic dependent lusitropic function of mouse heart. J Physiol. 2015 Sep 29. doi: 10.1113/JP271225. [Epub ahead of print] PMID: 26415898

  20. Flemming A, Huang QQ, Jin JP, Jumaa H, Herzog S. A Conditional Knockout Mouse Model Reveals That Calponin-3 Is Dispensable for 2Early B Cell Development. PLoS One. 2015 Jun 5;10(6):e0128385. PMID: 26046660

  21. Amarasinghe C, Jin JP. The Use of Affinity Tags to Overcome Obstacles in Recombinant Protein Expression and Purification. Protein Pept Lett. 2015 Jul 28. PMID: 26216265

  22. Feng HZ, Chen X, Malek M, Jin JP. Slow recovery of fatigue resistance in mouse soleus muscle corresponding to decreased mitochondrial function and a compensatory increase in type I slow fibers. Am J Physiol Cell Physiol. 2016 Jan 1;310(1):C27-40. Epub 2015 Oct 7. PMID: 26447205

  23. Sheng JJ, Jin JP. TNNI1, TNNI2 and TNNI3: Evolution, Regulation, and Protein Structure-Function Relationships. Gene, Gene. 2015 Oct 22. PMID: 26526134

  24. Sheng JJ, Feng HZ, Pinto JR, Wei H, Jin JP. Increases of desmin and α-actinin in mouse cardiac myofibrils as a response to diastolic dysfunction. J Mol Cell Cardiol. 2015 Oct 31. pii: S0022-2828(15)30104-8. PMID: 26529187

  25. Wei B, Jin JP. TNNT1, TNNT2, and TNNT3: Isoform genes, regulation, and structure-function relationships. Gene. 2016 Jan 13. pii: S0378-1119(16)00037-8. PMID: 26774798

  26. Jin JP. Evolution, Regulation, and Function of N-terminal Variable Region of Troponin T: Modulation of Muscle Contractility and Beyond. Int Rev Cell Mol Biol. 2016;321:1-28. PMID: 26811285

  27. Gunther LK, Feng HZ, Wei H, Raupp J, Jin JP, Sakamoto T. Effect of N-terminal extension of cardiac troponin I on the Ca2+ regulation of ATP-binding and ADP dissociation of myosin II in native cardiac myofibrils. Biochemistry. 2016 Mar 29;55(12):1887-97. doi: 10.1021/acs.biochem.5b01059. Epub 2016 Mar 14. PMID: 26862665

  28. Zhang T, Pereyra AS, Wang ZM, Birbrair A, Reisz JA, Files DC, Purcell L, Feng X, Messi ML, Feng H, Chalovich J, Jin JP, Furdui C, Delbono O. Calpain inhibition rescues troponin T3 fragmentation, increases Cav1.1, and enhances skeletal muscle force in aging sedentary mice. Aging Cell. 2016 Feb 19. PMID: 26892246

  29. Liu R, Jin JP. Calponin isoforms CNN1, CNN2 and CNN3: Regulators for actin cytoskeleton functionsi n smooth muscle and non-muscle cells. Gene. 2016 Mar 10. PMID: 26970176

  30. Amarasinghe CK, Hossain MM, Jin JP. Functional basis of three new recessive mutations of slow skeletal muscle troponin T found in non-Amish TNNT1 nemaline myopathies. Biochemistry. 2016 Jul 18. PMID: 27429059

  31. Huang QQ, Hossain MM, Sun W, Xing L, Pope RM, Jin JP. Deletion of calponin 2 in macrophages attenuates the severity of inflammatory arthritis in mice. Am J Physiol Cell Physiol. 2016 Aug 3:ajpcell.00331.2015. PMID: 27488671

  32. Liu R, Jin JP. Deletion of calponin 2 in macrophages alters cytoskeleton-based functions and attenuates the development of atherosclerosis. J Mol Cell Cardiol. 2016 Aug 26. pii: S0022-2828(16)30319-4. doi: 10.1016/j.yjmcc.2016.08.019. PMID: 27575021

  33. Usacheva EA, Jin JP, Peterson LR. Host response to Clostridium difficile infection: Diagnostics and detection. J Glob Antimicrob Resist. 2016 Sep 20;7:93-101. doi: 10.1016/j.jgar.2016.08.002. PMID: 27693863

  34. Mondal A, Jin JP. Protein Structure-Function Relationship at Work: Learning from Myopathy Mutations of the Slow Skeletal Muscle Isoform of Troponin T. Front Physiol. 2016 Oct 13;7:449. PMID: 27790152

  35. Hossain MM, Zhao G, Woo MS, Wang JH, Jin JP. Deletion of calponin 2 in mouse fibroblasts increases myosin II-dependent cell traction force. Biochemistry.  2016 Oct 13. PMID: 27733037

(underline denotes a current or previous graduate student)

Dr. Jin's Photo Galleries:
    (Please click on the thumbnails below for an expanded view)

Lab Personnel


Lab Staff

Down time

Hard at work

Symposium

Fall 2013 Cardiology Conference
 Cordoba, Argentina
soccer game after the conference

Dr. Jin with Javier and his son.
Cordoba Argentina
Research

Anatomy of Actin Thin Filament Regulatory System

Regulation of Troponin & Calcitonin

The N-Terminal Region
Regulates TnT Conformation
& Function

Evolution Linkage
of TnT & Tnl
  
Enhanced Phagpcytosis of
Neurophils & Macrophages
    
Lab publication gets cover
of JMCC
Books

Click on image to enlarge
link to publisher

Release Date: June 15, 2014
 
The Next Generation

Benjamin Nixon, Ph.D. 
Benjamin Nixon, of the Biomedical Sciences Graduate Program at The Ohio State University, successfully defended his dissertation titled "Effect of Post-translational Modification Crosstalk on Thin Filament Regulatory Function in Striated Muscle" on March 25, 2014.  His research was conducted under the mentorship of Brandon Biesiadecki, Ph.D., Assistant Professor of Physiology & Cell Biology at The Ohio State University.  Dr. Biesiadecki received his Ph.D. from Case Western Reserve in 2002, under the mentorship of Dr. Jin.

last update: 11/14/2016 by CRC.